Article
454 sequencing of pooled BAC clones on chromosome 3H of barley.
BMC genomics - 19 May 2011
Sato Kazuhiro, Motoi Yuka, Yamaji Nami, Yoshida Hideya
Abstract excerpt
BACKGROUND: Genome sequencing of barley has been delayed due to its large genome size (ca. 5,000 Mbp). Among the fast sequencing systems, 454 liquid phase pyrosequencing provides the longest reads and is the most promising method for BAC clones. Here we report the results of pooled sequencing of BAC clones selected with ESTs genetically mapped to chromosome 3H. RESULTS: We sequenced pooled barley BAC clones using...
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