Article
A sequence-ready physical map of barley anchored genetically by two million single-nucleotide polymorphisms.
Plant physiology - 1 Jan 2014
Ariyadasa Ruvini, Mascher Martin, Nussbaumer Thomas, Schulte Daniela, Frenkel Zeev, Poursarebani Naser, Zhou Ruonan, Steuernagel Burkhard, Gundlach Heidrun, Taudien Stefan, Felder Marius, Platzer Matthias, Himmelbach Axel, Schmutzer Thomas, Hedley Pete E, Muehlbauer Gary J, Scholz Uwe, Korol Abraham, Mayer Klaus F X, Waugh Robbie, Langridge Peter, Graner Andreas, Stein Nils
Abstract excerpt
Barley (Hordeum vulgare) is an important cereal crop and a model species for Triticeae genomics. To lay the foundation for hierarchical map-based sequencing, a genome-wide physical map of its large and complex 5.1 billion-bp genome was constructed by high-information content fingerprinting of almost 600,000 bacterial artificial chromosomes representing 14-fold haploid genome coverage. The resultant physical map...
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