Article
A high-density transcript linkage map of barley derived from a single population.
Heredity - 1 Aug 2009
Sato K, Nankaku N, Takeda K
Abstract excerpt
A high-resolution transcript linkage map of barley was created using a single doubled haploid (DH) mapping population, only 3'-end expressed sequence tags (ESTs) and only PCR-based assays. Cultivar 'Haruna Nijo' and an ancestral wild-form accession 'H602' were used as EST donors and crossing parents of the mapping population. Of the 10,366 primer sets developed from a non-redundant set of 3'EST sequences, 7700...
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