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Article

Cloning, sequencing, and characterization of the nucH gene encoding an extracellular nuclease from Aeromonas hydrophila JMP636.

Journal of bacteriology - 1 Jul 1996

Dodd H N, Pemberton J M

Abstract excerpt

An Escherichia coli clone expressing activity on DNase agar was obtained by cloning chromosomal DNA of Aeromonas hydrophila JMP636 into plasmid pUC19. Examination (of the clone's nuclease activity on a sodium dodecyl sulfate (SDS)-polyacrylamide gel containing DNA as a substrate revealed an activity band at approximately 100 kDa. Subsequently, subcloning localized the gene, designated nucH, to a 3.6-kb DNA...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 8682799
DOI: 10.1128/jb.178.13.3926-3933.1996
PMCID: PMC232655

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SDS

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Source receipt: pubmed, retrieved 7 Sept 2026.

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