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Article

Fully hydrated yeast cells imaged with electron microscopy.

Biophysical journal - 18 May 2011

Peckys Diana B, Mazur Peter, Gould Kathleen L, de Jonge Niels

Abstract excerpt

We demonstrate electron microscopy of fully hydrated eukaryotic cells with nanometer resolution. Living Schizosaccharomyces pombe cells were loaded in a microfluidic chamber and imaged in liquid with scanning transmission electron microscopy (STEM). The native intracellular (ultra)structures of wild-type cells and three different mutants were studied without prior labeling, fixation, or staining. The STEM images...

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Identifiers

PMID: 21575587
DOI: 10.1016/j.bpj.2011.03.045
PMCID: PMC3093553

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