Defining the transcriptome assembly and its use for genome dynamics and transcriptome profiling studies in pigeonpea (Cajanus cajan L.).

doi:10.1093/dnares/dsr007

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Article

Defining the transcriptome assembly and its use for genome dynamics and transcriptome profiling studies in pigeonpea (Cajanus cajan L.).

DNA research : an international journal for rapid publication of reports on genes and genomes - 1 Jun 2011

Dubey Anuja, Farmer Andrew, Schlueter Jessica, Cannon Steven B, Abernathy Brian, Tuteja Reetu, Woodward Jimmy, Shah Trushar, Mulasmanovic Benjamin, Kudapa Himabindu, Raju Nikku L, Gothalwal Ragini, Pande Suresh, Xiao Yongli, Town Chris D, Singh Nagendra K, May Gregory D, Jackson Scott, Varshney Rajeev K

Abstract excerpt

This study reports generation of large-scale genomic resources for pigeonpea, a so-called 'orphan crop species' of the semi-arid tropic regions. FLX/454 sequencing carried out on a normalized cDNA pool prepared from 31 tissues produced 494 353 short transcript reads (STRs). Cluster analysis of these STRs, together with 10 817 Sanger ESTs, resulted in a pigeonpea trancriptome assembly (CcTA) comprising of 127 754...

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PMID: 21565938
DOI: 10.1093/dnares/dsr007
PMCID: PMC3111231

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