Article
Next-generation sequencing for identification of candidate genes for Fusarium wilt and sterility mosaic disease in pigeonpea (Cajanus cajan).
Plant biotechnology journal - 1 May 2016
Singh Vikas K, Khan Aamir W, Saxena Rachit K, Kumar Vinay, Kale Sandip M, Sinha Pallavi, Chitikineni Annapurna, Pazhamala Lekha T, Garg Vanika, Sharma Mamta, Sameer Kumar Chanda Venkata, Parupalli Swathi, Vechalapu Suryanarayana, Patil Suyash, Muniswamy Sonnappa, Ghanta Anuradha, Yamini Kalinati Narasimhan, Dharmaraj Pallavi Subbanna, Varshney Rajeev K
Abstract excerpt
To map resistance genes for Fusarium wilt (FW) and sterility mosaic disease (SMD) in pigeonpea, sequencing-based bulked segregant analysis (Seq-BSA) was used. Resistant (R) and susceptible (S) bulks from the extreme recombinant inbred lines of ICPL 20096 × ICPL 332 were sequenced. Subsequently, SNP index was calculated between R- and S-bulks with the help of draft genome sequence and reference-guided assembly of...
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