Article
Influence of nicotine on the biological activity of rabbit osteoblasts.
Clinical oral implants research - 1 Mar 2011
Ma Li, Zwahlen Roger Arthur, Zheng Li Wu, Sham Mai Har
Abstract excerpt
OBJECTIVES: To assess the influence of nicotine on the proliferation and gene expression of osteogenic and angiogenic mediators of osteoblasts. MATERIAL AND METHODS: Rabbit primary osteoblasts were exposed to various concentrations of nicotine (0.001, 0.1 and 10 μmol/l). The cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. The gene expression of transforming growth...
Topics
- Animals
- Bone Morphogenetic Protein 2
- Cell Culture Techniques
- Cell Proliferation
- Coloring Agents
- Neovascularization, Physiologic
- Nicotine
- Osteoblasts
- Osteogenesis
- Phenotype
- Platelet-Derived Growth Factor
- Rabbits
- Reverse Transcriptase Polymerase Chain Reaction
- Tetrazolium Salts
- Thiazoles
- Time Factors
- Transforming Growth Factor beta
- Vascular Endothelial Growth Factor A