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Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens

1 Feb 1990

Abstract excerpt

The rotavirus gene segment coding for the major outer capsid glycoprotein vp7 was amplified directly from stool specimens by the polymerase chain reaction (PCR). Double-stranded RNA extracted from stool samples was used as the template for reverse transcription, which was followed immediately and in the same reaction mix with amplification, using the Taq polymerase. Various conditions were examined to optimize...

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PMID: 2155916
DOI: 10.1128/jcm.28.2.276-282.1990

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