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Detection of group B and C rotaviruses by polymerase chain reaction

1 Mar 1991

Abstract excerpt

We adapted the polymerase chain reaction (PCR) to detect the noncultivatable group B and C rotaviruses and introduced a simple and convenient technique to purify viral RNA from stool specimens. Double-stranded RNA present in stool extracts was purified by adsorption to hydroxyapatite and was used as the template for reverse transcription and polymerase amplification. Primer pairs specific for group B (gene 8) and...

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PMID: 1645368
DOI: 10.1128/jcm.29.3.519-523.1991

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