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Article

Detection and genotyping of human rotavirus VP4 and VP7 genes by reverse transcriptase PCR and reverse hybridization.

Journal of clinical microbiology - 1 Sept 2009

van Doorn Leen-Jan, Kleter Bernhard, Hoefnagel Evert, Stainier Isabelle, Poliszczak Annick, Colau Brigitte, Quint Wim

Abstract excerpt

Rotavirus infections can be diagnosed in stool samples by serological and molecular methods. We developed a novel reverse transcriptase PCR (RT-PCR) method for the amplification of rotavirus RNA and a reverse hybridization assay on a strip to detect amplimers and identify the specific G and P gen...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 19553575
DOI: 10.1128/JCM.00378-09
PMCID: PMC2738096

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-19553575-2026-09-04.1. Manifest publication-graph-cohort-2026-09-04.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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