Article
High-quality DNA sequence capture of 524 disease candidate genes.
Proceedings of the National Academy of Sciences of the United States of America - 19 Apr 2011
Shen Peidong, Wang Wenyi, Krishnakumar Sujatha, Palm Curtis, Chi Aung-Kyaw, Enns Gregory M, Davis Ronald W, Speed Terence P, Mindrinos Michael N, Scharfe Curt
Abstract excerpt
The accurate and complete selection of candidate genomic regions from a DNA sample before sequencing is critical in molecular diagnostics. Several recently developed technologies await substantial improvements in performance, cost, and multiplex sample processing. Here we present the utility of long padlock probes (LPPs) for targeted exon capture followed by array-based sequencing. We found that on average 92% of...
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