Article
Establishment of lymphotoxin beta receptor signaling-dependent cell lines with follicular dendritic cell phenotypes from mouse lymph nodes.
Journal of immunology (Baltimore, Md. : 1950) - 15 Oct 2006
Nishikawa Yumiko, Hikida Masaki, Magari Masaki, Kanayama Naoki, Mori Masaharu, Kitamura Hiroshi, Kurosaki Tomohiro, Ohmori Hitoshi
Abstract excerpt
Follicular dendritic cells (FDCs) have been shown to play a crucial role in the positive selection of high-affinity B cells that are generated by somatic hypermutation in germinal center (GC). Because of technical difficulties in preparing and maintaining pure FDCs, a role for FDCs in this complicated process has not been fully elucidated. In this study, we established a cell line designated as pFL that retained...
Topics
- Animals
- B-Cell Activating Factor
- B-Lymphocytes
- Cell Culture Techniques
- Cell Line
- Coculture Techniques
- Dendritic Cells, Follicular
- Flow Cytometry
- Gene Expression Profiling
- Germinal Center