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Detection of virulent Escherichia coli O157 strains using multiplex PCR and single base sequencing for SNP characterization.

Journal of applied microbiology - 1 Jun 2011

Haugum K, Brandal L T, Løbersli I, Kapperud G, Lindstedt B-A

Abstract excerpt

AIMS: To compare 167 Norwegian human and nonhuman Escherichia coli O157:H7/NM (nonmotile) isolates with respect to an A/T single nucleotide polymorphism (SNP) in the tir gene and to detect specific SNPs that differentiate STEC O157 into distinct virulence clades (1-3 and 8). METHODS AND RESULTS: We developed a multiplex PCR followed by single base sequencing for detection of the SNPs, and examined the association...

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PMID: 21447016
DOI: 10.1111/j.1365-2672.2011.05015.x

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