Article
Genetic features differentiating bovine, food, and human isolates of shiga toxin-producing Escherichia coli O157 in The Netherlands.
Journal of clinical microbiology - 1 Mar 2012
Franz Eelco, van Hoek Angela H A M, van der Wal Fimme J, de Boer Albert, Zwartkruis-Nahuis Ans, van der Zwaluw Kim, Aarts Henk J M, Heuvelink Annet E
Abstract excerpt
The frequency of Escherichia coli O157 genotypes among bovine, food, and human clinical isolates from The Netherlands was studied. Genotyping included the lineage-specific polymorphism assay (LSPA6), the Shiga-toxin-encoding bacteriophage insertion site assay (SBI), and PCR detection and/or subtyping of virulence factors and markers [stx1, stx(2a)/stx(2c), q21/Q933, tir(A255T), and rhsA(C3468G)]. LSPA6 lineage II...
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