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Article

Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔC expression strain.

BMC biotechnology - 25 Mar 2011

Noens Elke E, Williams Chris, Anandhakrishnan Madhankumar, Poulsen Christian, Ehebauer Matthias T, Wilmanns Matthias

Abstract excerpt

BACKGROUND: The non-pathogenic bacterium Mycobacterium smegmatis is widely used as a near-native expression host for the purification of Mycobacterium tuberculosis proteins. Unfortunately, the Hsp60 chaperone GroEL1, which is relatively highly expressed, is often co-purified with polyhistidine-tagged recombinant proteins as a major contaminant when using this expression system. This is likely due to a...

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Identifiers

PMID: 21439037
DOI: 10.1186/1472-6750-11-27
PMCID: PMC3076238

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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