Article
A heterogeneous tag-attachment to the homodimeric type 1 photosynthetic reaction center core protein in the green sulfur bacterium Chlorobaculum tepidum.
Biochimica et biophysica acta - 1 Jul 2011
Azai Chihiro, Kim Kwang, Kondo Toru, Harada Jiro, Itoh Shigeru, Oh-oka Hirozo
Abstract excerpt
The 6xHis-tag-pscA gene, which was genetically engineered to express N-terminally histidine (His)-tagged PscA, was inserted into a coding region of the recA gene in the green sulfur bacterium Chlorobaculum tepidum (C. tepidum). Although the inactivation of the recA gene strongly suppressed a homologous recombination in C. tepidum genomic DNA, the mutant grew well under normal photosynthetic conditions. The...
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