Article
The assembly of a multisubunit photosynthetic membrane protein complex: a site-specific spin labeling EPR spectroscopic study of the PsaC subunit in photosystem I.
Biochemistry - 23 Mar 2010
Jagannathan Bharat, Dekat Sarah, Golbeck John H, Lakshmi K V
Abstract excerpt
The assembly of the PsaC subunit in the photosystem I (PS I) complex was studied using site-specific spin labeling electron paramagnetic resonance (EPR) spectroscopic techniques. The binding was monitored from the perspective of a reporter spin label attached to either the native C34(C) or the engineered C75(C) residue of wild-type PsaC (PsaC(WT)). Three distinct stages of PsaC assembly were analyzed: unbound...
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