Development of a nonfluorescent multiplex semiquantitative polymerase chain reaction to confirm rearrangements detected by array-comparative genomic hybridization.

doi:10.1089/gtmb.2010.0144

Back to search

Article

Development of a nonfluorescent multiplex semiquantitative polymerase chain reaction to confirm rearrangements detected by array-comparative genomic hybridization.

Genetic testing and molecular biomarkers - 1 Jan 2000

Huin Vincent, Drouot Nathalie, Chambon Pascal, Le Meur Nathalie, Frébourg Thierry, Tosi Mario, Saugier-Veber Pascale

Abstract excerpt

Copy number variations in the human genome are commonly detected using genome-wide high-resolution screening methods such as array-comparative genomic hybridization. To confirm these copy number variations, we have used an assay based on a semiquantitative multiplex polymerase chain reaction (PCR) of short unlabeled fragments and performed on the microfluidic Bio-Rad Experion system. We have compared the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Genetic Variation

Share to Genome, Human

Share to Polymerase Chain Reaction

Share to Reproducibility of Results

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 8 closest

Development of a nonfluorescent multiplex semiquantitative polymerase chain reaction to confirm rearrangements detected by array-comparative genomic hybridization.PMID 21413874Read

Screening for genomic rearrangements by multiplex PCR/liquid chromatography.Semantic proximity Read

A method for accurate detection of genomic microdeletions using real-time quantitative PCRSemantic proximity Read

Detection and characterization of NF1 microdeletions by custom high resolution array CGH.Semantic proximity Read

Array-Based Comparative Genomic Hybridization (aCGH).Semantic proximity Read

Methods to detect and analyze copy number variations at the genome-wide and locus-specific levels.Semantic proximity Read

Progress in the detection of human genome structural variations.Semantic proximity Read

Microarray-based genomic DNA profiling technologies in clinical molecular diagnostics.Semantic proximity Read

Methods and strategies for analyzing copy number variation using DNA microarrays.Semantic proximity Read