Article
Fluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eIF3 complex.
PloS one - 7 Mar 2011
Roy Bijoyita, Copenhaver Gregory P, von Arnim Albrecht G
Abstract excerpt
BACKGROUND: Mutations in several subunits of eukaryotic translation initiation factor 3 (eIF3) cause male transmission defects in Arabidopsis thaliana. To identify the stage of pollen development at which eIF3 becomes essential it is desirable to examine viable pollen and distinguish mutant from wild type. To accomplish this we have developed a broadly applicable method to track mutant alleles that are not...
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