Article
Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana.
The Plant journal : for cell and molecular biology - 1 Nov 2007
Boavida Leonor C, McCormick Sheila
Abstract excerpt
Despite much effort, a robust protocol for in vitro germination of Arabidopsis thaliana pollen has been elusive. Here we show that controlled temperatures, a largely disregarded factor in previous studies, and a simple optimized medium, solidified or liquid, yielded pollen germination rates above 80% and pollen tube lengths of hundreds of microns, with both Columbia and Landsberg erecta (Ler) ecotypes. We found...
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