Article
Profiling of translatomes of in vivo-grown pollen tubes reveals genes with roles in micropylar guidance during pollination in Arabidopsis.
The Plant cell - 1 Feb 2014
Lin Shih-Yun, Chen Pei-Wei, Chuang Ming-Hsiang, Juntawong Piyada, Bailey-Serres Julia, Jauh Guang-Yuh
Abstract excerpt
Transcriptome profiling has been used to identify genes expressed in pollen tubes elongating in vitro; however, little is known of the transcriptome of in vivo-grown pollen tubes due to the difficulty of collecting pollen that is elongating within the solid maternal gynoecium. Using a pollen-specific promoter (ProLAT52) to generate epitope-tagged polysomal-RNA complexes that could be affinity purified, we...
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