Article
Multiple isoelectric variants of copper, zinc-superoxide dismutase from rat liver.
Archives of biochemistry and biophysics - 15 May 1990
Yano S
Abstract excerpt
Isoelectric variants of Cu,Zn-superoxide dismutase (Cu,Zn-SOD) have been reported to exist in various organs including rat liver. To elucidate the biochemical characteristics of the variants, rat liver Cu,Zn-SOD was purified and isolated into eight variants, i.e., pI 5.15, 4.88, 4.80, 4.75, 4.70, 4.65, 4.60, and 4.50. The pI 4.88 variant had the highest specific activity (4245 U/mg protein) and the highest yield...
Topics
- Animals
- Chromatography, DEAE-Cellulose
- Coloring Agents
- Genetic Variation
- Isoelectric Focusing
- Liver
- Male
- Rats
- Rats, Inbred Strains
- Specific Pathogen-Free Organisms
- Substrate Specificity