Article
A mutation that enhances synthesis of sigma 32 and suppresses temperature-sensitive growth of the rpoH15 mutant of Escherichia coli.
Journal of bacteriology - 1 Apr 1990
Yano R, Nagai H, Shiba K, Yura T
Abstract excerpt
The rpoH15 mutant cannot grow at or above 34 degrees C, because it produces an altered sigma 32 protein that is largely deficient in the transcription of the heat shock genes. Extragenic suppressor mutations (suhB) located at 55 min on the Escherichia coli chromosome endowed the mutant cell with the ability to grow at 40 degrees C and the inability to grow at 25 degrees C. One such mutation (suhB2), studied in...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Bacteriophage lambda
- Base Sequence
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Genotype
- Molecular Sequence Data
- Mutation
- Plasmids