A mutant sigma 32 with a small deletion in conserved region 3 of sigma has reduced affinity for core RNA polymerase.

Abstract excerpt

sigma 70, encoded by rpoD, is the major sigma factor in Escherichia coli. rpoD285 (rpoD800) is a small deletion mutation in rpoD that confers a temperature-sensitive growth phenotype because the mutant sigma 70 is rapidly degraded at high temperature. Extragenic mutations which reduce the rate of degradation of RpoD285 sigma 70 permit growth at high temperature. One class of such suppressors is located in rpoH,...

Topics

• Chromosome Deletion
• DNA-Directed RNA Polymerases
• Heat-Shock Proteins
• Mutation
• Sigma Factor
• Transcription, Genetic