Article
Analysis of post transcriptional regulation of SOX9 mRNA during in vitro chondrogenesis.
Tissue engineering. Part A - 1 Jul 2011
Tew Simon R, Clegg Peter D
Abstract excerpt
Marker genes are used to monitor chondrogenic differentiation, but little is known about the turnover of their mRNA during this process. We set out to measure the half life of mRNA encoding the transcription factor SOX9, an important marker of chondrocytic phenotype. We dedifferentiated human articular chondrocytes in monolayer culture before placing them in chondrogenic three-dimensional pellet cultures. At the...
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