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Rapid genetic mapping of ESTs using SNP pyrosequencing and indel analysis.

Cellular & molecular biology letters - 1 Jan 2002

Ching Ada, Rafalski Antoni

Abstract excerpt

We describe an effective systematic approach to genetic mapping of cDNA clones, including those obtained from EST sequencing. The EST of interest is first partially sequenced from the 3'-end. PCR primers which bracket the 3'-UTR segment of the cDNA are designed. The corresponding gene segment is amplified from the parents of the mapping population, using primers equipped with 3'- and 5'-extensions to facilitate...

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PMID: 12378241

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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