Article
Multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in Bacillus cereus batch cultures.
Biotechnology letters - 1 Jul 2011
Want Andrew, Hancocks Helen, Thomas Colin R, Stocks Stuart M, Nebe-von-Caron Gerhard, Hewitt Christopher J
Abstract excerpt
Based on two staining protocols, DiOC(6)(3)/propidium iodide (PI) and RedoxSensor Green (an indicator of bacterial reductase activity)/PI, multi-parameter flow cytometry and cell sorting has identified at least four distinguishable physiological states during batch cultures of Bacillus cereus. Furthermore, dependent on the position in the growth curve, single cells gave rise to varying numbers of colonies when...
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