Article
Sybr Green- and TaqMan-Based Quantitative PCR Approaches Allow Assessment of the Abundance and Relative Distribution of Frankia Clusters in Soils.
Applied and environmental microbiology - 1 Mar 2017
Ben Tekaya Seifeddine, Ganesan Abirama Sundari, Guerra Trina, Dawson Jeffrey O, Forstner Michael R J, Hahn Dittmar
Abstract excerpt
The nodule-forming actinobacterial genus Frankia can generally be divided into 4 taxonomic clusters, with clusters 1, 2, and 3 representing nitrogen-fixing strains of different host infection groups and cluster 4 representing atypical, generally non-nitrogen-fixing strains. Recently, quantitative PCR (qPCR)-based quantification methods have been developed for frankiae of clusters 1 and 3; however, similar...
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