Article
Structure-function analysis of the TibA self-associating autotransporter reveals a modular organization.
Infection and immunity - 1 May 2011
Côté Jean-Philippe, Mourez Michael
Abstract excerpt
Some enterotoxigenic Escherichia coli strains express the TibA adhesin/invasin, a multifunctional autotransporter that mediates the autoaggregation of bacteria, biofilm formation, adhesion to cultured epithelial cells, and invasion of these cells. To elucidate the structure-function relationship in TibA, we generated mutants by transposon-based linker scanning mutagenesis and by site-directed mutagenesis. Several...
Topics
- Adhesins, Escherichia coli
- Bacterial Adhesion
- Escherichia coli
- Immunoblotting
- Mutagenesis, Site-Directed
- Mutation
- Polymerase Chain Reaction
- Structure-Activity Relationship
