Article
Catalase activity of cytochrome C oxidase assayed with hydrogen peroxide-sensitive electrode microsensor.
Biochemistry. Biokhimiia - 1 Nov 2010
Bolshakov I A, Vygodina T V, Gennis R, Karyakin A A, Konstantinov A A
Abstract excerpt
An iron-hexacyanide-covered microelectrode sensor has been used to continuously monitor the kinetics of hydrogen peroxide decomposition catalyzed by oxidized cytochrome oxidase. At cytochrome oxidase concentration ~1 µM, the catalase activity behaves as a first order process with respect to peroxide at concentrations up to ~300-400 µM and is fully blocked by heat inactivation of the enzyme. The catalase (or,...
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