Article
Engineered Tobacco mosaic virus mutants with distinct physical characteristics in planta and enhanced metallization properties.
Virus research - 1 Apr 2011
Kadri Anan, Maiss Edgar, Amsharov Nadja, Bittner Alexander M, Balci Sinan, Kern Klaus, Jeske Holger, Wege Christina
Abstract excerpt
Tobacco mosaic virus mutants were engineered to alter either the stability or surface chemistry of the virion: within the coat protein, glutamic acid was exchanged for glutamine in a buried portion to enhance the inter-subunit binding stability (E50Q), or a hexahistidine tract was fused to the surface-exposed carboxy terminus of the coat protein (6xHis). Both mutant viruses were expected to possess specific metal...
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