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Article

Direct fluorescence detection of VirE2 secretion by Agrobacterium tumefaciens.

PloS one - 1 Jan 2017

Yaakov Noga, Barak Yoav, Pereman Idan, Christie Peter J, Elbaum Michael

Abstract excerpt

VirE2 is a ssDNA binding protein essential for virulence in Agrobacterium tumefaciens. A tetracysteine mutant (VirE2-TC) was prepared for in vitro and in vivo fluorescence imaging based on the ReAsH reagent. VirE2-TC was found to be biochemically active as it binds both ssDNA and the acidic secretion chaperone VirE1. It was also biologically functional in complementing virE2 null strains transforming Arabidopsis...

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PMID: 28403156
DOI: 10.1371/journal.pone.0175273
PMCID: PMC5389803

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