Article
In vitro assembly of cowpea chlorotic mottle virus from coat protein expressed in Escherichia coli and in vitro-transcribed viral cDNA.
Virology - 10 Mar 1995
Zhao X, Fox J M, Olson N H, Baker T S, Young M J
Abstract excerpt
The small spherical plant virus, cowpea chlorotic mottle virus (CCMV), provides an ideal system to examine spherical virus assembly. We have modified the CCMV in vitro assembly system to produce virions from coat protein expressed in Escherichia coli and viral RNA transcribed in vitro from full-l...
Topics
- Base Sequence
- Bromovirus
- Capsid
- DNA Primers
- DNA, Complementary
- DNA, Viral
- Escherichia coli
- Gene Expression
- Image Processing, Computer-Assisted
- Microscopy, Electron
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Plants
- Protein Conformation
- Transcription, Genetic