Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Amplification ratio control system for copy number variation genotyping.

Nucleic acids research - 1 Apr 2011

Guthrie Philip A I, Gaunt Tom R, Abdollahi Mohammed R, Rodriguez Santiago, Lawlor Debbie A, Smith George Davey, Day Ian N M

Abstract excerpt

We describe a generic design for ratiometric analysis suitable for determination of copy number variation (CNV) class of a gene. Following two initial sequence-specific PCR priming cycles, both ends of both amplicons (one test and one reference) in a duplex reaction, are all primed by the same universal primer (UP). Following each amplification denaturation step, the UP target and its reverse complement (UP') in...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Pairing
Share to Chemokines, CC

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Copy Number Variations

Share to DNA Primers

Share to Gene Dosage

Share to Haptoglobins

Share to Nucleic Acid Denaturation

Share to Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 21300641
DOI: 10.1093/nar/gkr046
PMCID: PMC3082892

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Amplification ratio control system for copy number variation genotyping.PMID 21300641Read ↗

CNV analysis using TaqMan copy number assays.Semantic proximity Read ↗

Quantitative analysis of copy number variants based on real-time LightCycler PCR.Semantic proximity Read ↗

Probe-specific mixed-model approach to detect copy number differences using multiplex ligation-dependent probe amplification (MLPA).Semantic proximity Read ↗

Development and validation of a high throughput, closed tube method for the determination of haemoglobin alpha gene (HBA1 and HBA2) numbers by gene ratio assay copy enumeration-PCR (GRACE-PCR).Semantic proximity Read ↗

Complement C4 Gene Copy Number Variation Genotyping by High Resolution Melting PCR.Semantic proximity Read ↗

A quantitative assay to detect α-thalassemia deletions and triplications using multiplex nested real-time quantitative polymerase chain reaction.Semantic proximity Read ↗

Optimized Assessment of qPCR-Based Vector Copy Numbers as a Safety Parameter for GMP-Grade CAR T Cells and Monitoring of Frequency in PatientsSemantic proximity Read ↗

Self-Internal-Reference Probe System for Control-Free Quantification of Mutation Abundance.Semantic proximity Read ↗

Statistical tools for transgene copy number estimation based on real-time PCRSemantic proximity Read ↗

Detection of genetic variations in coagulopathy-related genes using ramified rolling circle amplification.Semantic proximity Read ↗

RTPrimerDB: the real-time PCR primer and probe database, major update 2006.Semantic proximity Read ↗

Elimination of locus-specific inter-individual variation in quantitative PCR.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.