Article
Elimination of locus-specific inter-individual variation in quantitative PCR.
BioTechniques - 1 Dec 2004
Shi Jinxiu, Liu Qiang, Nguyen Vu Q, Sommer Steve S
Abstract excerpt
Robust dosage PCR (RD-PCR), a duplex and quantitative PCR, detects large heterozygous deletions and duplications in genomic DNA samples. RD-PCR amplifies an endogenous internal control and a target locus. Two of six RD-PCR assays behaved anomalously due to lower yields specific to the targets. The variability was eliminated by heat treatment of the genomic DNA samples in 2x TE solution at 90 degrees C for 10 min....
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