Article
RapA, Escherichia coli RNA polymerase SWI/SNF subunit-dependent polyadenylation of RNA.
Biochemistry - 29 Mar 2011
Richmond Michael, Pasupula Raghavendra R, Kansara Seema G, Autery Joshua P, Monk Brent M, Sukhodolets Maxim V
Abstract excerpt
In this work, we describe RapA-dependent polyadenylation of model RNA substrates and endogenous, RNA polymerase-associated nucleic acid fragments. We demonstrate that the Escherichia coli RNA polymerase obtained through the classic purification procedure carries endogenous RNA oligonucleotides, which, in the presence of ATP, are polyriboadenylated in a RapA-dependent manner by an accessory poly(rA) polymerase....
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