Article
Extracellular beta-galactosidase activity of a Fibrobacter succinogenes S85 mutant able to catabolize lactose.
Applied and environmental microbiology - 1 Dec 1990
Javorsky P, Lee S F, Gibbins A M, Forsberg C W
Abstract excerpt
Fibrobacter succinogenes S85 is unable to grow with lactose as the source of carbohydrate, although it does exhibit low beta-galactosidase (EC 3.2.1.23) activity. Spontaneous mutants of strain S85 able to grow on lactose were isolated after spreading cells on a chemically defined agar medium with lactose as the carbohydrate source. A lactose-catabolizing isolate, designated L2, exhibited a sodium dodecyl...
Topics
- Animals
- Bacteria, Anaerobic
- Blotting, Western
- Chromatography, High Pressure Liquid
- Electrophoresis, Polyacrylamide Gel
- Hydrogen-Ion Concentration
- Lactose
- Microscopy, Phase-Contrast
- Mutation
- Rumen
- beta-Galactosidase