Article
[Cloning of the RIB7 gene encoding the riboflavin synthase of the yeast Pichia guilliermondii].
Genetika - 1 Jun 1993
Logvinenko E M, Stasiv Iu Z, Zlochevskiĭ M L, Voronovskiĭ A Ia, Beburov M Iu, Shavlovskiĭ G M
Abstract excerpt
The RIB7 gene encoding the enzyme of the final stage of riboflavin biosynthesis in Pichia guilliermondii--riboflavin synthase was cloned on the pFL38 shuttle vector as the Sau3A fragment of the chromosomal DNA of about 4 kb. The HindIII fragment of 1.4 kb was subcloned from the hybrid plasmid pFR7 obtained onto the pUC18 plasmid. The plasmid pR7 thus constructed transform Escherichia coli ribB-45 mutant cells...
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