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Article

Vulnerability of the cysteine-less proton-coupled folate transporter (PCFT-SLC46A1) to mutational stress associated with the substituted cysteine accessibility method.

Biochimica et biophysica acta - 1 Apr 2011

Zhao Rongbao, Shin Daniel Sanghoon, Goldman I David

Abstract excerpt

The proton-coupled transporter (PCFT) mediates intestinal folate absorption and folate transport from blood across the choroid plexus. The membrane topology of PCFT has been defined using the substituted cysteine accessibility method; an intramolecular disulfide bond between the Cys 66 and 298 residues, in the first and fourth extracellular loops, respectively, is present but not essential for function. The...

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Identifiers

PMID: 21256110
DOI: 10.1016/j.bbamem.2011.01.008
PMCID: PMC3062655

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Vulnerability of the cysteine-less proton-coupled folate transporter (PCFT-SLC46A1) to mutational stress associated with the substituted cysteine accessibility method.PMID 21256110Read ↗

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