Article
Scanning and negative-staining electron microscopy of protoplast regeneration of a wild-type and two chitin synthase mutants in the pathogenic yeast Candida glabrata.
Journal of electron microscopy - 1 Jan 2011
Namiki Yuichi, Ueno Keigo, Mitani Hiroki, Virtudazo Eric V, Ohkusu Misako, Shimizu Kiminori, Kawamoto Susumu, Chibana Hiroji, Yamaguchi Masashi
Abstract excerpt
Protoplast regeneration of a wild-type and two mutant strains of Candida glabrata defective in CHS3 homologues encoding class IV chitin synthase in Saccharomyces cerevisiae was examined by scanning and negative-staining electron microscopy. In the wild-type strain, small particles and short filaments appeared on the protoplast surface at 10 min, filamentous materials covered the entire surface of the protoplast...
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