Article
Molecular comparison of a nonhemolytic and a hemolytic phospholipase C from Pseudomonas aeruginosa.
Journal of bacteriology - 1 Oct 1990
Ostroff R M, Vasil A I, Vasil M L
Abstract excerpt
Pseudomonas aeruginosa produces two secreted phospholipase C (PLC) enzymes. The expression of both PLCs is regulated by Pi. One of the PLCs is hemolytic, and one is nonhemolytic. Low-stringency hybridization studies suggested that the genes encoding these two PLCs shared DNA homology. This information was used to clone plcN, the gene encoding the 77-kilodalton nonhemolytic PLC, PLC-N. A fragment of plcN was used...
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