Article
ATP independent type IB topoisomerase of Leishmania donovani is stimulated by ATP: an insight into the functional mechanism.
Nucleic acids research - 1 Apr 2011
Sengupta Souvik, Ganguly Agneyo, Roy Amit, Bosedasgupta Somdeb, D'Annessa Ilda, Desideri Alessandro, Majumder Hemanta K
Abstract excerpt
Most type IB topoisomerases do not require ATP and Mg(2+) for activity. However, as shown previously for vaccinia topoisomerase I, we demonstrate that ATP stimulates the relaxation activity of the unusual heterodimeric type IB topoisomerase from Leishmania donovani (LdTOP1L/S) in the absence of Mg(2+). The stimulation is independent of ATP hydrolysis but requires salt as a co-activator. ATP binds to LdTOP1L/S and...
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