Article
Transmutation of a heme protein.
Proceedings of the National Academy of Sciences of the United States of America - 15 Jul 1993
Barker P D, Ferrer J C, Mylrajan M, Loehr T M, Feng R, Konishi Y, Funk W D, MacGillivray R T, Mauk A G
Abstract excerpt
Residue Asn57 of bovine liver cytochrome b5 has been replaced with a cysteine residue, and the resulting variant has been isolated from recombinant Escherichia coli as a mixture of four major species: A, BI, BII, and C. A combination of electronic spectroscopy, 1H NMR spectroscopy, resonance Raman spectroscopy, electrospray mass spectrometry, and direct electrochemistry has been used to characterize these four...
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