Article
Transmutation of a heme protein.
Proceedings of the National Academy of Sciences of the United States of America - 15 Jul 1993
Barker P D, Ferrer J C, Mylrajan M, Loehr T M, Feng R, Konishi Y, Funk W D, MacGillivray R T, Mauk A G
Abstract excerpt
Residue Asn57 of bovine liver cytochrome b5 has been replaced with a cysteine residue, and the resulting variant has been isolated from recombinant Escherichia coli as a mixture of four major species: A, BI, BII, and C. A combination of electronic spectroscopy, 1H NMR spectroscopy, resonance Rama...
Topics
- Animals
- Cattle
- Cysteine
- Cytochromes b5
- Genetic Variation
- Magnetic Resonance Spectroscopy
- Mutagenesis, Site-Directed
- Mutation
- Peptide Fragments
- Protoporphyrins
- Recombinant Proteins
- Spectrophotometry
- Spectrum Analysis, Raman