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Article

Genetic tagging, cloning, and DNA sequence of the Synechococcus sp. strain PCC 7942 gene (gnd) encoding 6-phosphogluconate dehydrogenase.

Journal of bacteriology - 1 Jul 1990

Broedel S E, Wolf R E

Abstract excerpt

A genetic approach was used for the cloning of the Synechococcus sp. strain PCC 7942 (Synechococcus strain R2) gnd gene which encodes 6-phosphogluconate dehydrogenase (6PGD). A restriction map of the gnd locus was prepared by Southern analysis using the Escherichia coli gene as a heterologous probe. The Synechococcus strain R2 gene was genetically tagged by restriction site-specific insertion of the nptII gene of...

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Identifiers

PMID: 2113917
DOI: 10.1128/jb.172.7.4023-4031.1990
PMCID: PMC213388

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