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Article

Genetic tagging, cloning, and DNA sequence of the Synechococcus sp. strain PCC 7942 gene (gnd) encoding 6-phosphogluconate dehydrogenase.

Journal of bacteriology - 1 Jul 1990

Broedel S E, Wolf R E

Abstract excerpt

A genetic approach was used for the cloning of the Synechococcus sp. strain PCC 7942 (Synechococcus strain R2) gnd gene which encodes 6-phosphogluconate dehydrogenase (6PGD). A restriction map of the gnd locus was prepared by Southern analysis using the Escherichia coli gene as a heterologous probe. The Synechococcus strain R2 gene was genetically tagged by restriction site-specific insertion of the nptII gene of...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Cyanobacteria
DNA
Escherichia coli
Gene Library
Genes, Plant
Molecular Sequence Data
Mutation
Phosphogluconate Dehydrogenase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Restriction Mapping

Sequence Homology, Nucleic Acid

Source

Open in PubMed Open full text

Identifiers

PMID: 2113917
DOI: 10.1128/jb.172.7.4023-4031.1990
PMCID: PMC213388

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2113917-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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