Article
The Bacillus subtilis phoAIV gene: effects of in vitro inactivation on total alkaline phosphatase production.
Gene - 30 Nov 1990
Kapp N V, Edwards C W, Chesnut R S, Hulett F M
Abstract excerpt
A degenerative oligodeoxyribonucleotide probe deduced from the first 19 amino acids of the mature alkaline phosphatase IV (APase IV) protein was used to clone a DNA fragment internal to the coding region of the phoAIV gene of Bacillus subtilis. An insertional mutation was constructed in the phoAIV locus using the integrative plasmid, pJM103, containing the cloned DNA fragment. The strain with the interrupted...
Topics
- Alkaline Phosphatase
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- Blotting, Western
- Chromosome Mapping
- Cloning, Molecular
- Genes, Bacterial
- Molecular Sequence Data
- Mutation