Article
Identification of distinct NAD-linked hydrogenase protein species in mutants and nickel-deficient wild-type cells of Alcaligenes eutrophus H16.
European journal of biochemistry - 20 May 1990
Hornhardt S, Schneider K, Friedrich B, Vogt B, Schlegel H G
Abstract excerpt
By crossed immunoelectrophoresis with antibodies against the NAD-linked hydrogenase the presence of three hydrogenase protein species was demonstrated in crude extracts of Alcaligenes eutrophus H16. Protein 1 (antigen 1) exhibited NAD-reducing activity and was shown to be identical with the nativ...
Topics
- Alcaligenes
- Immunoassay
- Immunodiffusion
- Immunoelectrophoresis, Two-Dimensional
- Mutation
- Nickel
- Oxidoreductases
- Phenotype
