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Development of 1-Cell Embryos from Different Strains of Mice in CZB Medium1

1 Mar 1990

Abstract excerpt

One-cell embryos from several different strains of mice have been cultured to the blastocyst stage in CZB medium. CZB medium can be used to culture CF1 x B6SJLF1/J 1-cell embryos to the blastocyst stage provided glucose is introduced into the medium on Day 3 of culture. The amount of glucose required for embryo development was titrated using a concentration range of 5.5 to 49.5 mM. With the exception of the...

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PMID: 2111184
DOI: 10.1095/biolreprod42.3.432

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