Article
Some effects of genotype and composition of the culture medium on the development of mouse zygotes in vitro.
Reproduction, fertility, and development - 1 Jan 1993
Du Z F, Wales R G
Abstract excerpt
The effects of EDTA and the presence of glucose and glutamine in CZB medium on the development of mouse zygotes of different genotype were investigated. Although 30-80% of zygotes (depending on the cross) passed the 2-cell stage in EDTA-free medium, the addition of a low concentration of EDTA was necessary in these experiments to obtain blastocysts in culture. In reciprocal crosses between outbred (Qs), inbred...
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