Article
Domain architecture of the DRpp29 protein and its interaction with the RNA subunit of Dictyostelium discoideum RNase P.
Biochemistry - 21 Dec 2010
Stamatopoulou Vassiliki, Toumpeki Chrisavgi, Tzakos Andreas, Vourekas Anastassios, Drainas Denis
Abstract excerpt
Dictyostelium discoideum nuclear RNase P is a ribonucleoprotein complex that displays similarities with its counterparts from higher eukaryotes such as the human enzyme, but at the same time it retains distinctive characteristics. In the present study, we report the molecular cloning and interaction details of DRpp29 and RNase P RNA, two subunits of the RNase P holoenzyme from D. discoideum. Electrophoretic...
Topics
- Blotting, Northern
- Dictyostelium
- Electrophoretic Mobility Shift Assay
- Immunoprecipitation
- Mutation
- Protein Binding
- Protein Subunits
- RNA, Catalytic
- Ribonuclease P